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diff --git a/academic/primer3/README b/academic/primer3/README index 2ef54f3a5a..51977f2f12 100644 --- a/academic/primer3/README +++ b/academic/primer3/README @@ -1,11 +1,14 @@ -Primer3 is a widely used program for designing PCR primers. Primer3 -can also design hybridization probes and sequencing primers. PCR is -used for many different goals. Consequently, primer3 has many -different input parameters that you control and that tell primer3 +Primer3 is a widely used program for designing PCR primers (PCR = +"Polymerase Chain Reaction"). PCR is an essential and ubiquitous tool +in genetics and molecular biology. Primer3 can design hybridization +probes and sequencing primers. + +PCR is used for many different goals. Consequently, primer3 has many +different input parameters that you control and that tell primer3 exactly what characteristics make good primers for your goals. For details and citation: -Steve Rozen and Helen J. Skaletsky (2000) Primer3 on the WWW for -general users and for biologist programmers. In: Krawetz S, Misener S -(eds) Bioinformatics Methods and Protocols: Methods in Molecular +Steve Rozen and Helen J. Skaletsky (2000) Primer3 on the WWW for +general users and for biologist programmers. In: Krawetz S, Misener S +(eds) Bioinformatics Methods and Protocols: Methods in Molecular Biology. Humana Press, Totowa, NJ, pp 365-386 |