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| author |   Petar Petrov | 2011-09-04 17:59:26 +0200 |
|---|---|---|
| committer |   Niels Horn | 2011-09-04 17:59:26 +0200 |
| commit | f82ed85b019ac2ef2166beaf031a2d32947f3011 (patch) | |
| tree | 4ec77684901fac11a1fb9052acd61e46d09d8903 /academic/perlprimer/README | |
| parent | 776abd8e685f6f33553a143fa83645178842eb75 (diff) | |
| download | slackbuilds-f82ed85b019ac2ef2166beaf031a2d32947f3011.tar.gz | |
academic/perlprimer: Added (Design primers for PCR and sequencing)
Signed-off-by: Niels Horn <niels.horn@slackbuilds.org>
Diffstat (limited to 'academic/perlprimer/README')
| -rw-r--r-- | academic/perlprimer/README | 26 |
1 files changed, 26 insertions, 0 deletions
diff --git a/academic/perlprimer/README b/academic/perlprimer/README new file mode 100644 index 0000000000..12917e35e9 --- /dev/null +++ b/academic/perlprimer/README @@ -0,0 +1,26 @@ +PerlPrimer is a free, open-source GUI application written in Perl +that designs primers for standard PCR, bisulphite PCR, real-time PCR +(QPCR) and sequencing. It aims to automate and simplify the process +of primer design. + +PerlPrimer's current features include the following: + +- Calculation of possible primer-dimers +- Retrieval of genomic or cDNA sequences from Ensembl (including both + sequences automatically for QPCR) +- Ability to BLAST search primers using the NCBI server or a local + server +- Results can be saved or optionally exported in a tab-delimited + format that is compatible with most spreadsheet applications. +- ORF and CpG island detection algorithms +- Ability to add cloning sequences to primers, automatically adjusted + to be in-frame +- QPCR primer design without manual intron-exon boundary entry + +For details and citation: +Marshall OJ. PerlPrimer: cross-platform, graphical primer design for +standard, bisulphite and real-time PCR. Bioinformatics 2004 +20(15):2471-2472 + +This requires perl-tk and libwww-perl. +For Real Time PCR, spidey is required. |