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diff --git a/academic/primer3/README b/academic/primer3/README index 51977f2f12..3b57af159d 100644 --- a/academic/primer3/README +++ b/academic/primer3/README @@ -1,14 +1,24 @@ Primer3 is a widely used program for designing PCR primers (PCR = -"Polymerase Chain Reaction"). PCR is an essential and ubiquitous tool -in genetics and molecular biology. Primer3 can design hybridization -probes and sequencing primers. +"Polymerase Chain Reaction"). PCR is an essential and ubiquitous tool in +genetics and molecular biology. Primer3 can design hybridization probes +and sequencing primers. PCR is used for many different goals. Consequently, primer3 has many different input parameters that you control and that tell primer3 exactly what characteristics make good primers for your goals. -For details and citation: -Steve Rozen and Helen J. Skaletsky (2000) Primer3 on the WWW for -general users and for biologist programmers. In: Krawetz S, Misener S -(eds) Bioinformatics Methods and Protocols: Methods in Molecular -Biology. Humana Press, Totowa, NJ, pp 365-386 +Primer3 picks primers for PCR reactions, considering as criteria: +* oligonucleotide melting temperature, size, GC content and primer-dimer + possibilities, +* PCR product size +* positional constraints within the source (template) sequence +* possibilities for ectopic priming (amplifying the wrong sequence) +* many other constraints. + +All of these criteria are user-specifiable as constraints, and some are +specifiable as terms in an objective function that characterizes an +optimal primer pair. + +manual online: https://primer3.org/manual.html + +For details and citation see "References" in the package's doc folder. |