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Diffstat (limited to 'academic/perlprimer/README')
| -rw-r--r-- | academic/perlprimer/README | 18 |
1 files changed, 9 insertions, 9 deletions
diff --git a/academic/perlprimer/README b/academic/perlprimer/README index bb7ab97f96..70a8c1ca8d 100644 --- a/academic/perlprimer/README +++ b/academic/perlprimer/README @@ -1,21 +1,21 @@ -PerlPrimer is a free, open-source GUI application written in Perl -that designs primers for standard PCR, bisulphite PCR, real-time PCR -(QPCR) and sequencing. It aims to automate and simplify the process -of primer design. +PerlPrimer is a free, open-source GUI application written in Perl +that designs primers for standard PCR, bisulphite PCR, real-time PCR +(QPCR) and sequencing. It aims to automate and simplify the process +of primer design. PerlPrimer's current features include the following: - Calculation of possible primer-dimers -- Retrieval of genomic or cDNA sequences from Ensembl (including both +- Retrieval of genomic or cDNA sequences from Ensembl (including both sequences automatically for QPCR) -- Ability to BLAST search primers using the NCBI server or a local +- Ability to BLAST search primers using the NCBI server or a local server -- Results can be saved or optionally exported in a tab-delimited +- Results can be saved or optionally exported in a tab-delimited format that is compatible with most spreadsheet applications. - ORF and CpG island detection algorithms -- Ability to add cloning sequences to primers, automatically adjusted +- Ability to add cloning sequences to primers, automatically adjusted to be in-frame -- QPCR primer design without manual intron-exon boundary entry +- QPCR primer design without manual intron-exon boundary entry An optional run-time dependency is spidey, for Real Time PCR. |