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author | Petar Petrov | 2018-12-24 03:11:11 +0100 |
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committer | Willy Sudiarto Raharjo | 2018-12-24 03:11:11 +0100 |
commit | 5a3aecffdca70b25e44a246ed1b601b01a6674d8 (patch) | |
tree | 4511294e4326572c49c197c239c859b1dfee5c20 /academic/diamond/README | |
parent | f6b1cc61cacf8d462a563b885065902a78d72fa7 (diff) | |
download | slackbuilds-5a3aecffdca70b25e44a246ed1b601b01a6674d8.tar.gz |
academic/diamond: Added (A sequence aligner).
Signed-off-by: Willy Sudiarto Raharjo <willysr@slackbuilds.org>
Diffstat (limited to 'academic/diamond/README')
-rw-r--r-- | academic/diamond/README | 33 |
1 files changed, 33 insertions, 0 deletions
diff --git a/academic/diamond/README b/academic/diamond/README new file mode 100644 index 0000000000..a5c66d0988 --- /dev/null +++ b/academic/diamond/README @@ -0,0 +1,33 @@ +DIAMOND is a sequence aligner for protein and translated DNA searches, +designed for high performance analysis of big sequence data. The key +features are: + +- Pairwise alignment of proteins and translated DNA at 500x-20,000x + speed of BLAST. +- Frameshift alignments for long read analysis. +- Low resource requirements and suitable for running on standard + desktops or laptops. +- Various output formats, including BLAST pairwise, tabular and XML, + as well as taxonomic classification. + +To now run an alignment task, we assume to have a protein database file +in FASTA format named `nr.faa` and a file of DNA reads that we want to +align named `reads.fna`. + +In order to set up a reference database for DIAMOND, the `makedb` +command needs to be executed with the following command line: + + $ diamond makedb --in nr.faa -d nr + +This will create a binary DIAMOND database file with the specified name +(`nr.dmnd`). The alignment task may then be initiated using the `blastx` +command like this: + + $ diamond blastx -d nr -q reads.fna -o matches.m8 + +The output file here is specified with the `–o` option and named +`matches.m8`. By default, it is generated in BLAST tabular format. + +Publication: +Buchfink B, Xie C, Huson DH, "Fast and sensitive protein alignment using +DIAMOND", Nature Methods 12, 59-60 (2015). doi:10.1038/nmeth.3176 |